Introduction. In the recent years, three-dimensional (3D) bioprinted hydrogels has been developed and tested for regenerative medicine due to their ability to mimic the anatomical structures [1,2]. Various bioinks that contain polymeric biomaterials, especially natural polymers have been prepared and tested[3] due to their biocompatibility and favorable environment for cell attachment and...
Introduction
Hydrogel fillings have been successful in enhancing artificial conduits for nerve injuries, but the ideal hydrogel is yet to be found. Its discovery is impeded by an overflow of available products as well as a lack of in vitro experiments that fully analyze the effects of these hydrogels on cells crucial for nerve regeneration.
Methodology
This study investigated various...
The recent emergence of nanotechnology has provided a new therapeutic modality in case of silver nanoparticles. Dressings containing silver form the basis for the treatment of burns and wounds, either acute or chronic ones. The aim of the study was to examine silver release from the different wound dressings: commercially available (Atrauman Ag, Aquacel Ag) and experimental (FKDP-AgNPs) using...
A novel decellularized bone matrix was established by the supercritical carbon dioxide (SCCO2) extraction technology. The most commonly employed surgical technique is bone grafting, to enhance bone regeneration in orthopaedic surgery. The current study investigated the role of SCCO2 -decellularized bone matrix (scDBM) as bio-scaffold seeded with allogeneic adipose-derived mesenchymal stem...
Introduction:Hydrogels are biomaterials that possess appropriate physicochemical and mechanical properties enabling the formation of a three-dimensional, stable structure used in tissue engineering, providing an environment conducive to the survival and proliferation of cells. The cytotoxicity test is performed to assess the overall toxicity of developed materials. Evaluation of the...
*Purpose: Tissue engineering is an interdisciplinary field involving activities based on two interrelated areas: developing new methods to repair, regenerate and replace damaged tissues and organs. Currently, the most suitable materials for bioprinting of living constructs are biomaterials based on decellularized extracellular matrix (ECM). In this researches, we made two independent...
Urochordates are the closest invertebrate relative to humans and commonly referred to as tunicates, a name ascribed to their leathery outer “tunic”. The tunic is the outer covering of the organism which functions as the exoskeleton and is rich in carbohydrates and proteins. Invasive or fouling tunicates pose a great threat to the indigenous marine ecosystem and governments spend several...
INTRODUCTION: Hypertrophic chondrocytes mediate the enduring pathway for endochondral bone formation. However, challenges in tissue-engineered bone constructs include functionality under the mechanical environment inside in vivo conditions. We aim to fabricate a mechanically stable Silk fibroin (SF) carrier incorporating decellularized cartilage derived extracellular matrix (CD-ECM) and...
Introduction: The main aim of tissue engineering is to design biological substitutes in order to enhance and restore damaged tissues or organs, and biomaterials for fabricating the scaffolds play a crucial role. Collagen (COL), the main part of the ECM and a group of structural proteins which contributes to the extracellular scaffolding, was considered for scaffolds preparation. Studies have...
Introduction
Craniofacial bone defects can be caused from birth defect anomalies and road traffic accidents and affect vital functions such as chewing, swallowing, speaking, and breathing1. Autografts are the current gold standard for treating these defects but suffer from limited tissue available for harvest, long operation times, and donor site morbidity. Tissue-engineering has been known...
Introduction
Osteoarthritis (OA) of articular cartilage is a progressive and debilitating disease, often necessitating a total joint replacement. Between 2005 and 2015 the worldwide prevalence of OA increased by 32.9 % (from 17.9 to 23.7 million cases) [1]. There is therefore a demand for early stage interventions to stave off or delay replacement surgery. A potential treatment is a...
Tissue engineering has undergone a great revolution in terms of the strategies, technologies and materials it uses (1). In this way, much more complex approximations have been proposed achieving a notorious improvement of the treatment of injuries as frequent as tendinopathies. In this sense, 3D bioprinting could help for the development of scaffolds whose biological or mechanical...
Introduction
A major drawback of most chemo- and radiotherapeutic cancer treatments is their toxicity to the ovaries, meaning they may affect fertility and/or endocrine function of female patients. The state-of-the-art method nowadays is the cryopreservation of the ovaries. However, with transplantation of ovarian tissue after the end of cancer treatment there is always the risk to also...
The injured spinal cord (SC) generates a unique and complex pathophysiology which presents a multifaceted challenge for repair. Broadly, normally supportive astroglia become reactive following injury and form a glial scar. This scar in turn inhibits recovery by releasing an impenetrable inhibitive extracellular matrix (ECM), which prevents axonal regrowth. Tissue engineering approaches aim to...
Introduction:
Breast cancer is the most frequent cancer among women. New therapeutic approaches need to be introduced to develop an effective cancer therapy, which could inhibit, or reverse cancer spread to distant locations. The most prevalent cancer treatment method is resection, during which the tumor with the adjacent tissue (margin) is removed. In such case, patient-tailored hydrogel...
Introduction: Urinary bladder regeneration using tissue engineering is one of the major challenges of reconstructive urology. Commonly used urinary bladder reconstruction techniques that utilize gastrointestinal tissues, are associated with many complications. One of the most promising alternative seems to be the use of bladder acellular matrix (BAM). The use of BAM in clinical practice...
In this study, we utilized the decellularized extracellular matrix (ECM) under the ultrasonication to obtain the ultrasonic decellularized ECM (UdECM). In SDS-PAGE studies, the results demonstrated that UdECM was mainly composed of type I collagen. The mechanical strength increased with the increase of concentration of UdECM in rheology studies. In addition, the scanning electron microscope...
INTRODUCTION: Liver diseases are becoming a significant public medical burden[1]. To overcome the lack of donor livers and high cost of therapy, this project aims to exploit a long-term culture system that maintains hepatocyte specific functions [2] and cell viability for liver regeneration using self-assembled peptide hydrogels to mimic the normal hepatic extracellular matrix (ECM). Previous...
(1) Background: The aim of the present study was the biocompatibility analysis of a novel xenogeneic vascular graft material (PAP) based on native collagen won from porcine aorta using the subcutaneous implantation model up to 120 days post implantationem. As a control, an already commercially available collagen-based vessel graft (XenoSure®) based on bovine pericardium was used. Another focus...
Introduction: Excessive muscle damage does not regenerate on its own, so research is being conducted to regenerate the muscle in tissue engineering. In this study, myoblast-patch that mimics muscle fiber was generated and characterized in vitro test.
Method: To generate myoblast-patch, scaffolds were fabricated with biocompatible materials using electrospinning. Two groups of scaffolds were...
Biocomposites made of poly(propylene carbonate) (PPC), a carbon dioxide (CO2) derived polymer, and a renewable polysaccharide are promising materials for several applications such as biomaterials and packaging. However, most of PPC-based biocomposites exhibit low mechanical and optical properties due to the inherent incompatibility between PPC and most other polymers. In this work, a novel...
Introduction: Damage of the sclerocorneal limbus may lead to limbal stem cell deficiency (LSCD), loss of corneal transparency, and blindness. Keratoplasty is the standard treatment for corneal transplantation disorders, but it is contraindicated in cases with LSCD or limbal damage1, which are very difficult to manage. Generation of a biological substitute of the sclerocorneal limbus could...
Although oral formulations of anticancer chemotherapies are clinically available, the therapeutic action relies on drug absorption, being inevitably accompanied with systemic side effects. It is thus desirable to develop oral therapy systems for the local treatment of colon cancers featured with highly selective delivery and minimized systemic drug absorption. The present study demonstrates...
Introduction: Electrospun wound dressings are emerging as promising candidates in the field of wound care since they present a similar architecture to natural extracellular matrix (ECM). Their composition can include both synthetic and natural polymers, the latter enabling a biological other than structural mimicking of ECM. Due to the high surface-to-volume ratio, electrospun matrices can...
Hydrogel materials are widely used in medicine and tissue engineering due to their similarity to the natural tissues of the human body. They are well known for their high biocompatibility and swelling - the ability to absorb large amounts of water. These properties determine the possibility of the use of hydrogels in the regeneration of cartilage tissue. In order to enhance regeneration...
Title: HYDROGELS FOR 3D EXTRUSION PRINTING OF GRADIENT SCAFFOLDS
Rency Geevarghese1, Joanna Żur-Pińska1, Małgorzata Włodarczyk-Biegun1,2 *
1Biofabrication and Bio-Instructive Materials, Biotechnology Center, The Silesian University of Technology, B. Krzywoustego 8, 44-100 Gliwice, Poland.
2Polymer Science, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747...
Introduction: Even though conventionally prepared octacalcium phosphate and collagen composite (OCPcol) has exhibited excellent bone regeneration with angiogenesis (1, 2) and has already been commercialized for treating bone defects (3), reproducible appositional bone formation with OCPcol has never been achieved. Recently, it was developed double concentration of OCP/Col with applying liquid...
The use of xeno-free (animal origin-free) hydrogels to support the growth of cells significantly increased in the recent years. The advantage of these materials in comparison with animal derivates is the reproducibility of structural, mechanical and biological properties and, additionally, overcoming of potential immunological complications and disease transmission in vivo. Many novel...
Introduction: In the last two decades research has been focused on the development of biologically-derived matrices for reconstructive medicine[1]. Decellularized tissues from a xenogeneic donor represent a growth-friendly environment for cells of different species, including human, lacking most of the immunogenic components[2]. Decellularized porcine liver resembles the human counterpart and...
Natural scaffolds derived from living tissue have been used in clinical applications. They provide similar architecture and composition to the target tissue and can be remodelled as desired. However, they still have some drawbacks as there is a risk of disease transmission and may require additional surgery. Therefore, extracellular matrix (ECM) based biohybrid scaffolds have been developed...
Supercritical CO2 (SCCO2) technology was used to eliminate the cells, fat and non-collagenous materials while preserving a native collagen scaffold as a biomedical device for bone defects. We used porcine matrix bones with 3D CAD/CAM carving to reconstruct a personalized, wide range and complex-shaped bone. In oral cancer patients upto 30–50% of need mandibulectomy and autologous fibula...
We proposed a new formation for fibrous mat fabrication from derivatives of polyvinyl alcohol (PVA) and gelatin (Gela) through horseradish peroxidase mediated crosslinking which provides sustain release of methylprednisolone (MP) as a therapeutic approach for SCI regeneration. The polymeric solution of PVA and Gela conjugated with phenolic moieties (PVAPh & GelaPh respectively) containing...
Introduction: Mesenchymal stromal cells (MSCs) have the potential to respond to injury and aid in repair and regeneration of damaged tissues. Bone marrow and adipose tissue are the major sources of MSCs for clinical applications. Previous studies in animal models suggested that the regenerative activity of aged stem cells/MSCs can be enhanced by exposure to biomimetic microenvironments. The...
Heart valve diseases have become one of the main causes for mortality worldwide. To increase the life expectancy of these patients, surgical replacements using mechanical or biological heart valve implants are most commonly used. However, mechanical implants entail the lifelong intake of anticoagulation medication and biological implants exhibit a limited lifetime of 10-20 years. In addition,...
Introduction: Several scaffolds have been introduced for urethral tissue engineering. However, acellular human urethral scaffold harvested from deceased donors may provide significant advantages in comparison to synthetic, composite or other biological scaffolds. The aim of this study was to develop a protocol for decellularization of human urethra that preserves substantial components of...
